Lnc001209 Participates in aluminium-induced apoptosis of PC12 cells by regulating PI3K-AKT-mTOR signalling pathway.

Aluminium (Al) is a common environmental neurotoxin, but the molecular mechanism underlying its toxic effects remains unclear. Many studies have shown that aluminium exposure leads to increased neuronal apoptosis. This study aimed to investigate the mechanisms and signalling pathways involved in aluminium exposure-induced neuronal apoptosis. The results showed a decrease in the number of PC12 cells and changes in cell morphology in the aluminium maltol exposure group. The viability of PC12 cells decreased gradually with increasing of exposure doses, and the apoptosis rate increased. The expression of Lnc001209 decreased gradually with an increase in the aluminium exposure dose. After transfection of Lnc001209 siRNA in aluminium-exposed PC12 cells, the protein expression levels of p-Akt Ser473, p-Akt Thr308, p-P85 Tyr467, p-mTOR Ser2448 and CD36 were increased. RNA pull-down MS showed that Lnc001209 interacts with the CD36 protein. Expression of the CD36 protein was increased in PC12 cells exposed to aluminium. The results of the CD36 intervention experiment showed that the protein expression levels of p-Akt Ser473, p-Akt Thr308, p-P85 Tyr467, and p-mTOR Ser2448 likely increased after CD36 overexpression. In addition, the phosphorylation level of AKT had the most significant increase. The enhancement of p-Akt activity promotes neuronal apoptosis.

Interaction between aluminum exposure and ApoEε4 gene on cognitive function of in-service workers.

The occurrence and development of cognitive impairment, the early stage of AD, may be affected both by factors of environmental (aluminum exposure) and genetic (ApoEε4 gene). But whether there is an interaction between the two factors on cognitive function is still unknown. To explore the interaction between the two factors on cognitive function of in-service workers. A total of 1121 in-service workers in a large aluminum factory were investigated in Shanxi Province. Cognitive function was assessed by the Mini-mental State Examination (MMSE), the clock-drawing test (CDT), the Digit Span Test (DST, including DSFT and DSBT), the fuld object memory evaluation (FOM), and the verbal fluency task (VFT). The plasma-Al (p-Al) concentrations were measured by inductively coupled plasma-mass spectrometry (ICP-MS) as an internal exposure indicator, and the participants were divided into four Al exposure groups according to the quartile of p-Al concentrations, namely Q1, Q2, Q3, and Q4. ApoE genotype was determined by Ligase Detection Reaction (LDR). The multiplicative model was fitted using non-conditional logistic regression and additive model was fitted using crossover analysis to analyze the interaction between p-Al concentrations and the ApoEε4 gene. Finally, a dose-response relationship between p-Al concentrations and cognitive impairment was observed, with the p-Al concentrations increased, cognitive function performance gradually becomes worse (Ptrend＜0.05), and the risk of cognitive impairment gradually increases (Ptrend＜0.05), mainly in executive/visuospatial impairment, auditory memory impairment (particularly the working memory impairment). And ApoEε4 gene may be a risk factor for cognitive impairment, while no association between the ApoEε2 gene and cognitive impairment is observed. Additionally, an additive but no multiplicative interaction between p-Al concentrations and ApoEε4 gene is observed, and when the two factors work together, the risk of cognitive impairment further increased, of which 44.2% can be attributed to the interaction effect.

Simultaneous effects of aluminum exposure on the homeostasis of essential metal content in rat brain and perturbation of gut microbiota.

The theory of the brain-gut axis has confirmed that gut microbiota and metabolites are involved in the progression of neurodegenerative diseases through multiple pathways. However, few studies have highlighted the role of gut microbiota in cognitive impairment induced by aluminum (Al) exposure and its correlations with the homeostasis of essential metal content in the brain. To explore the relationship between alterations in the content of essential metals in the brain and relative abundance changes in gut microbiota induced by Al exposure, the Al, zinc (Zn), copper (Cu), iron (Fe), chromium (Cr), manganese (Mn), and cobalt (Co) content level in the hippocampus, olfactory bulb, and midbrain tissue were measured by inductively coupled plasma mass spectrometry (ICP-MS) methods after Al maltolate was intraperitoneally injected every other day for exposed groups. Then the unsupervised principal coordinates analysis (PCoA) and linear discriminant analysis effect size (LEfSe) were used to analyze the relative abundance of the gut microbiota community and the structure of the gut microbiome. Finally, the correlations between gut microbiota composition and essential metal content in the different exposure groups were explored by using the Pearson correlation coefficient method. Based on the results, we indicated that the content of Al in the hippocampus, olfactory bulb, and midbrain tissue was increased and then decreased with the increasing exposure duration, with peaks occurring between 14 and 30 days. Concomitantly, Al-exposure decreased the Zn, Fe, and Mn levels in these tissues. 16 S rRNA gene sequencing results indicated that significant differences in the intestinal microbial community structure at the phylum, family, and genus levels were found in the Day 90 exposed group compared with the Day 7 exposed group. Ten enriched species in the exposed group were identified as markers at the three levels. Furthermore, ten bacteria at the genus level were identified to have a significantly strong correlation (r = 0.70-0.90) with Fe, Zn, Mn, and Co.

In-situ remediation of phosphogypsum in a cement-free pathway: Utilization of ground granulated blast furnace slag and NaOH pretreatment.

In-situ remediating phosphogypsum (PG) for cemented paste backfill (CPB) in the contaminated site is economic management for promoting sustainable developments in the phosphate industry. This study concerns the combined use of NaOH pretreatment and ground-granulated blast furnace slag (GGBFS) additives to promote the solidification/stabilization of PG with a lower carbon footprint pathway. According to physico-chemical analyses, the NaOH pretreatment effectively removed approximately 95% of F within the PG, which may originally be present as sparingly soluble fluorides or coexisting with silicates. The micro mineralogical characterization illustrates that the pretreatment can accelerate the early age hydration, with more hydration products observed, including calcium silicate hydrates and ettringite, effective F and P retention candidates. Whereas the incorporation of GGBFS plays an essential role in promoting the generation of additional cement hydrates at the following stages. The macro mechanical performance analysis indicates that the mixtures of pretreated-PG-OPC-GGBFS exhibit an excellent mechanical performance satisfying the design criteria. Subsequent elemental mapping and toxicity characteristic leaching procedures demonstrate that this combined approach has a competitive F and P immobilization ability compared to the typical OPC binder and individual GGBFS addition. The newly formed phases effectively controlled the concentration of F and P through adsorption, incorporation, or encapsulation. Objectively, the proposed methodology can be a promising candidate pathway for extrapolating the in-situ immobilization of PG. This study opens up new perspectives for synergetically recycling PG and GGBFS in a profitable and low carbon footprint way.

miR-200a-3p Regulates PRKACB and Participates in Aluminium-Induced Tau Phosphorylation in PC12 Cells.

Aluminium (Al) is an environmental neurotoxin that humans are widely exposed to, but the molecular mechanism of its toxic effects is not fully understood. Many studies have shown that exposure to Al can cause abnormal phosphorylation of the tau protein that is believed as one of pathological features of Alzheimer's disease. Increasing evidence indicates that microRNAs (miRNAs) may be involved in the pathological processes of neurodegenerative diseases and are potential regulatory factors for related target genes. Phosphorylation at Ser-133 of cAMP response element-binding protein (CREB) is one of the major pathways of CREB activation, and phosphorylation at this site is controlled by protein kinase A (PKA). The catalytic subunit of PKA, cAMP-dependent protein kinase catalytic subunit beta (PRKACB), phosphorylates CREB. The target gene prediction software TargetScan showed that PRKACB was one of the target mRNAs of miR-200a-3p. The purpose of this study was to investigate whether miR-200a-3p regulates the PKA/CREB pathway by targeting PRKACB and leads to abnormal phosphorylation of the tau protein in nerve cells. The results showed that Al exposure increased the expression level of miR-200a-3p, and miR-200a-3p increased the expression of targeted downregulated PRKACB, and then decreased the PKA/CREB signalling pathway activity, leading to abnormal hyperphosphorylation of tau.

Experimental Investigation on Red Mud from the Bayer Process for Cemented Paste Backfill.

Red mud is a by-product of alumina production, and its disposal can have severe environmental consequences. This study experimentally investigates the feasibility of using red mud from the Bayer process for cemented paste backfill (CPB). Different binders and activators were used to improve the mechanical properties, water resistance, and environmental behaviors of red mud-based CPB. In addition, water immersion tests were introduced, for the first time, to evaluate the water resistance of CPB. Furthermore, the environmental behaviors of red mud-based CPB were investigated by conducting leaching experiments. The results showed that the red-mud specimens had an unconfined compressive strength (UCS) of less than 0.2 MPa and disintegrated after being immersed in water. Different binders significantly improved the mechanical properties of red mud-based CPB. In addition, the specimens with different binders showed excellent water resistance, and the softening coefficient of CPB with different binders could exceed 0.7 after being cured for 28 days. The binders exhibited a substantial inhibitory effect on the leaching of hazardous substances in red mud under the solidification and stabilization effects. The leaching concentration of hexavalent chromium, selenium, fluoride, arsenic, lead, and vanadium was reduced by more than 70%. Therefore, this study provides an effective method for the environmental-friendly and large-scale utilization of red mud from the Bayer process.

The Slope Safety, Heavy Metal Leaching, and Pollutant Diffusion Prediction Properties under the Influence of Unclassified Cemented Paste Backfill in an Open Pit.

Open-pit unclassified cemented paste backfilling (OPUCPB) methods have not only addressed the disposal problems of tailings but also eliminated geological hazards of high and steep open pit slopes and created conditions for ecological restoration of the open pit in the future. In this paper, slope safety simulations, heavy metal leaching, groundwater monitoring, and pollutant diffusion predictions were examined to evaluate the slope safety pattern and environmental protection enabled by OPUCPB. The results showed that: (1) The safety factor of the open pit slope was proportional to the height of OPUCPB operation. Under the condition of seismic force and seepage field, the safety factor of slope B was increased from 1.188 to 1.574 by OPUCPB. (2) The toxic and harmful components in tailings were significantly stabilized by the OPUCPB. Under the conditions of acid leaching and water leaching, the quality of the leaching solution met the requirements of the class III limit of groundwater (GB/T14848-2017). (3) The monitoring results of groundwater quality around the open pit showed that the OPUCPB had no effect on groundwater, and the water quality met the requirements of the class III limit of groundwater (GB/T14848-2017). (4) Considering the diffusion prediction of pollutants and groundwater under extreme conditions, it was found that the pollution process is slow, and the shortest time required for pollutants to reach the standard limit is 232 d at a distance of 50 m from the leakage point. Therefore, the influence of OPUCPB can be controlled, and this method can achieve improved reclamation of open pits and safety treatment of tailings. It was worth popularizing and applying in mining enterprises.

Highly-efficient fluoride retention in on-site solidification/stabilization of phosphogypsum: Cemented paste backfill synergizes with poly-aluminum chloride activation.

Phosphogypsum (PG) is a massively generated hazardous by-product in the phosphorus industry. Large-scale, efficient, profitable on-site recycling is an emerging topic for promoting sustainable phosphorus circularity and mitigating potential human exposure. In this work, we integrated a green and low-cost additive polymeric aluminum chloride (PAC) into the binder design of PG immobilization. The overall experimental results illustrate that the incorporation of PAC can efficiently promote the cement hydration reaction, with amorphous phases increased from 25.9 wt% (control group) to 27.5 wt% (with 2 g/L PAC). The macro-investigations indicate that the PAC optimized the porosity and mechanical properties of specimens, facilitating a mechanically stable solidified matrix for extrapolating its field engineering application. The detailed micrographs and elemental mapping demonstrate that apart from co-existing with the hydration products, the PAC agent plays a role in the immobilization of fluoride. Herein, the combined optimization enhanced the fluoride retention capacity due to the precipitated additional hydration products, comparable encapsulation, and high adsorption ability of PAC agents. Therefore our design of PAC-augmented binders can open up a new field of PG on-site solidification/stabilization application that ensures efficient fluoride retention in a technically feasible and financially profitable methodology.

Environmental Benefit Assessment of Blended Cement with Modified Granulated Copper Slag.

This study aimed to investigate the environmental impact of modified granulated copper slag (MGCS) utilization in blended cement production at a representative cement plant in China. Sensitivity analysis was performed on the substance inputs, and the life cycle impact assessment (LCIA) model was applied. A detailed comparative analysis was conducted of the environmental impact of cement production in other studies, and ordinary Portland cement production at the same cement plant. Results showed that calcination has the largest contribution impact of all the impact categories, especially in causing global warming (93.67%), which was the most prominent impact category. The life cycle assessment (LCA) result of blended cement was sensitive to the chosen LCIA model and the depletion of limestone and energy. In this study, producing blended cement with MGCS effectively mitigated the environmental impact for all the selected impact categories. Results also show a reduction in abiotic depletion (46.50%) and a slight growth (6.52%) in human toxicity. The adoption of MGCS in blended cement would therefore generally decrease the comprehensive environmental impact of cement, which contributes to the development of sustainable building materials.

Involvement of Mitophagy in Primary Cultured Rat Neurons Treated with Nanoalumina.

The aim of this study was to explore the influence of the neurotoxicity of nanoalumina on primarily cultured neurons. Normal control, particle size control, aluminum, micron-alumina, and nanoalumina at 50-nm and 13-nm particle sizes were included as subjects to evaluate the level of apoptosis, necrosis, and autophagy in primarily cultured neurons and further explore the mitophagy induced by nanoalumina. The results demonstrated that nanoalumina could induce neuronal cell apoptosis, necrosis, and autophagy, among which autophagy was the most notable. When the autophagy inhibitor was added to the nanoalumina-treated group, it significantly downregulated the protein expression levels of Beclin-1 and LC3II/LC3. Observation under a transmission electron microscope and a fluorescence microscope revealed mitophagy characteristics induced by nanoalumina. Additionally, the neurotoxicological effects induced by nanoalumina were more significant than those induced by aluminum and in a particle size-dependent manner.

A study on cognitive impairment of mice exposed to nano-alumina particles by nasal drip.

BACKGROUND: As an emerging nanomaterial, nano-alumina is widely used in chemical engineering, food and medicine due to its special physical and chemical properties, and its potential health hazards have attracted attention. OBJECTIVE: Aim of this study is to understanding the effect and possible mechanism of nano-alumina on cognitive function in mice. METHODS: Male healthy ICR mice were randomly assigned and given nasal drops of saline, nano-alumina (different doses) and micro-alumina for 30 days, respectively. Morris water maze test, step down test and open field test were used to detect learning and memory ability. Blood brain permeability was observed by immunofluorescence staining and lanthanum nitrate tracing, histopathological abnormalities in mice hippocampus was observed by thionine staining, the final determination of oxidative stress level in brain tissue was measured by using oxidative stress index detection kit and the level of LC3-Ⅱ and Caspase-3, 8, 9 proteins were detected by western blot. RESULTS: In the cerebral cortex of mice exposed to nano-alumina particles, lanthanum nitrate particles adhered to vascular endothelial cells, and the expression of ZO-1 and Occuldin decreased and morphology was disordered; most neurons in hippocampus CA3 region showed balloon-like swelling and degeneration, nucleoli disappeared and apical dendrites broke; mice exposed to nano-alumina, the escape latency in Morris water maze increased compared with the control group(P < 0.05),and the residence time in the original platform quadrant shortened significantly(P < 0.05);the platform latency was significantly shortened and the number of errors increased in the step down test compared with the control group; the residence time in the center of mice the nano-alumina treated was significantly increased in open field test (P < 0.05). CONCLUSION: The nano-alumina particles could be transported into the central nervous system via blood-brain barrier and olfactory bulb, impair learning and memory function in mice, which is more serious than the micro-alumina particles. The apoptosis of mice neurons caused by nano-alumina particles maybe due to the mixed neurotoxic effect of oxidative stress and the elemental toxicity of aluminum itself.

Resistance Loss in Cemented Paste Backfill Pipelines: Effect of Inlet Velocity, Particle Mass Concentration, and Particle Size.

Cemented paste backfill (CPB), a technology placing the solid waste into mined-out stopes in the mine through pipeline transportation, has been widespread all over the world. The resistance loss is an important parameter for pipeline transport, which is significantly affected by the slurry characteristics. However, the coupling effect of inlet velocity (IV), particle mass concentration (PMC), and particle size (PS) has not been well evaluated and diagnosed. Hence, the CFD-based three-dimensional network simulation of CPB slurry flow in an L-shaped pipe at different combinations of the three parameters was developed using COMSOL Multiphysics software, and the findings were validated through a loop experiment. The results show that increasing IV and reducing PS will contribute to the homogeneity of the slurry in the pipeline, while the PMC presents little effect. The pipe resistance loss is positively correlated with IV and PMC and negatively correlated with PS. The sensitivity to the three parameters is IV > PS > PMC. In particular, the resistance loss is minimal at IV of 1.5 m/s, PMC of 72%, and PS of 1000 um. The calculation model of resistance loss regressed from simulation presented a high accuracy with an error of 8.1% compared with the test results. The findings would be important for the design of the CPB pipeline transportation and provide guidance in the selection of transfer slurry pumps, prepreparation of backfill slurry, and pipe blockage, which will improve the safety and economic level of a mine.

Hydration and Mechanical Properties of Blended Cement with Copper Slag Pretreated by Thermochemical Modification.

The application of granulated copper slag (GCS) to partially replace cement is limited due to its low pozzolanic activity. In this paper, reconstituted granulated copper slag (RGCS) was obtained by adding alumina oxide (Al2O3) to liquid copper slag. Blended cement pastes were formulated by a partial substitute for ordinary Portland cement (OPC) with the RGCS (30 wt%). The pozzolanic activity, mechanical development, and the microstructure were characterized. The results show that 5-10 wt% Al2O3 contributes to the increase in magnetite precipitation in RGCS. The addition of Al2O3 alleviates the inhibition of C3S by RGCS and accelerates the dissociation of RGCS active molecules, thus increasing the exothermic rate and cumulative heat release of the blended cement pastes, which are the highest in the CSA10 paste with the highest Al2O3 content (10 wt%) in RGCS. The unconfined compressive strength (UCS) values of blended cement mortar with 10 wt% Al2O3 added to RGCS reach 27.3, 47.4, and 51.3 MPa after curing for 7, 28 and 90 d, respectively, which are the highest than other blended cement mortars, and even exceed that of OPC mortar at 90 d of curing. The pozzolanic activity of RGCS is enhanced with the increase in Al2O3 addition, as evidenced by more portlandite being consumed in the CSA10 paste, forming more C-S-H (II) gel with a higher Ca/Si ratio, and a more compact microstructure with fewer pores than other pastes. This work provided a novel, feasible, and clean way to enhance the pozzolanic activity of GCS when it was used as a supplementary cementitious material.

Gene Selection in a Single Cell Gene Space Based on D-S Evidence Theory.

If the samples, features and information values in a real-valued information system are cells, genes and gene expression values, respectively, then for convenience, this system is said to be a single cell gene space. In the era of big data, people are faced with high dimensional gene expression data with redundancy and noise causing its strong uncertainty. D-S evidence theory excels at tackling the problem of uncertainty, and its conditions to be met are weaker than Bayesian probability theory. Therefore, this paper studies the gene selection in a single cell gene space to remove noise and redundancy with D-S evidence theory. The distance between two cells in each gene is first defined. Then, the tolerance relation is established according to the defined distance. In addition, the belief and plausibility functions to grasp the uncertainty of a single cell gene space are introduced on the basis of the tolerance classes. Statistical analysis shows that they can effectively measure the uncertainty of a single cell gene space. Furthermore, several gene selection algorithms in a single cell gene space are presented using the proposed belief and plausibility. Finally, the performance of the proposed algorithm is compared to other algorithms on some published single-cell data sets. Experimental results and statistical tests show that the classification and clustering performance of the presented algorithm not only exceeds the other three state-of-the-art algorithms, but also its gene reduction rate is very high.

Necrostatin-1 Relieves Learning and Memory Deficits in a Zebrafish Model of Alzheimer's Disease Induced by Aluminum.

Aluminum (Al) is considered one of the environmental risk factors for Alzheimer's disease (AD). The present study aims to establish a zebrafish AD model induced by Al and explore if necrostation-1 (Nec-1), a specific inhibitor of necroptosis, is effective in relieving learning and memory deficits in the zebrafish AD models. We treated adult zebrafish with aluminum trichloride at various doses for 1 month, followed by a T-maze test to evaluate learning and memory performance. Al concentration, levels of acetylcholine (Ach), and AD-related protein and gene expression in the brain tissue were evaluated in the zebrafish AD models. Our results demonstrated that in the brain tissue of Al-treated zebrafish, Al accumulated, Ach levels decreased, and AD-related genes and proteins increased. As a result, the learning and memory performance of Al-treated zebrafish was impaired. This suggested that a zebrafish AD model was established. To test the effect of Nec-1 on the zebrafish AD model, we added Nec-1 into the culture medium of the Al-treated adult zebrafish. The results demonstrated that Nec-1 could relive the learning and memory deficits, enhance Ach levels and the numbers of neural cells, and impact necroptosis-related gene expression. We concluded that Nec-1 could reverse Al-induced learning and memory impairment and had potential theoretical value in the zebrafish AD model.

The rheological, mechanical and heavy metal leaching properties of cemented paste backfill under the influence of anionic polyacrylamide.

Anionic polyacrylamide (APAM) has widely been employed in backfill mining to accelerate the sedimentation of fine tailings particles and increase the concentration of tailings slurry. However, APAM inevitably remains in thickened tailings, leading to a nonnegligible influence on the rheological, mechanical, and heavy metal leaching properties of tailings-based cemented paste backfill (CPB). In an effort to solve these issues, the influences of APAM on CPB properties were examined in the present study. Experimental tests such as rheology, uniaxial compressive strength (UCS), toxicity leaching, and microscopy were conducted. The results showed that the presence of APAM first significantly increased the yield stress and viscosity of CPB slurry. APAM slightly improved the early UCS of CPB curing for 7 days but hindered the UCS development of samples cured for 28 days. Moreover, the presence of APAM restrained the hydration reaction, reduced the amounts of hydrated products, increased pore size, and loosed the microstructure of the test samples. Finally, the addition of APAM effectively reduced the leaching of Ag and As, while incremented that of Cu and slightly affected the leaching of Ba. In sum, these findings look promising for the safe production and environmental protection of the mining industry.

Aluminum inhibits non-amyloid pathways via retinoic acid receptor.

BACKGROUND: Aluminium neurotoxicity has been widely confirmed and mainly manifests as cognitive impairment. Al3+ can inhibit the expression of ADAM10, a key enzyme of the nonamyloid pathway, but its mechanism of toxicity has not been fully elucidated. Studies have shown that RARs can regulate ADAM10 expression. METHODS: We explored whether Al3+ affects the expression of ADAM10 through RARs, thereby affecting the nonamyloid pathway. RESULTS: Al3+ reduced the expressions of RARα, RARß and ADAM10. The expression levels of the RARα, RARß and ADAM10 proteins were upregulated in the RA group compared with the control group. In the RA + 200 µmol Al(mal)3 group, the downregulation of RARα, RARß and ADAM10 was weaker than that of the 200 µmol Al(mal)3 group, which indicated that RA participated in and upregulated the expression of ADAM10 through RARα and RARß. CONCLUSION: Al3+ inhibits ADAM10 expression through RARα and RARß and results in a decrease in the nonamyloid pathway.

In Situ Remediation of Phosphogypsum with Water-Washing Pre-Treatment Using Cemented Paste Backfill: Rheology Behavior and Damage Evolution.

The accumulation of original phosphogypsum (OPG) has occupied considerable land resources, which have induced significant environmental problems worldwide. The OPG-based cemented paste backfill (OCPB) has been introduced as a promising solution. In this study, a water-washing pre-treatment was used to purify OPG, aiming to optimize the transport performance and mechanical properties of backfills. The overall results proved that in treated phosphogypsum-based cemented paste backfill (TCPB), the altered particle size distribution can alleviate the shear-thinning characteristic. The mechanical properties were significantly optimized, of which a maximum increase of 183% of stress value was observed. With more pronounced AE signals, the TCPB samples demonstrated better residual structures after the ultimate strength values but with more unstable cracks with high amplitude generated during loading. Principal component analysis confirmed the adverse effects of fluorine and phosphorus on the damage fractal dimensions. The most voluminous hydration products observed were amorphous CSH and ettringite. The interlocked stellate clusters may be associated with the residual structure and the after-peak AE events evident in TCPB, indicate that more significant stress should be applied to break the closely interlocked stitches. Ultimately, the essential findings in this experimental work can provide a scientific reference for efficient OPG recycling.

Neurodevelopmental toxicity of alumina nanoparticles to zebrafish larvae: Toxic effects of particle sizes and ions.

The aim of this study was to explore the mechanism of neurodevelopmental toxicity of alumina nanoparticles (AlNPs) on zebrafish larvae, specifically, the toxic effects of AlNPs of different particle sizes and of dissolved aluminum ions. AlNPs with sizes of 13 nm (13 nm-Al) and 50 nm (50 nm-Al) were used as the main research objects; while nanocarbon particles with sizes of 13 nm (13 nm-C) and 50 nm (50 nm-C) as particle-size controls; and an aluminum chloride solution (Al3+) as an ion control. Zebrafish embryos were exposed to different treatments from 6 h post-fertilization (hpf) to 168 hpf. Deformities were observed at different time points. Neurodevelopmental behavior tests were carried out, and oxidative stress responses and transcriptional alterations in autophagy-related genes were assessed. Malformations occurred in the 13 nm-Al, 50 nm-Al, and Al3+ treated groups at different developmental stages of zebrafish larval, but no malformations were observed in the 13 nm-C or 50 nm-C groups. In addition, the average speed, distance travelled and thigmotaxis in zebrafish larvae decreased in the AlNPs treated group, and the effects were related to the particle sizes. Furthermore, increases in the oxidative stress response and autophagy-related genes expression were also related to the particle sizes of AlNPs as well. In conclusion, the mechanism underlying the neurodevelopmental toxicity of AlNPs on zebrafish larvae mainly depended on the size of the nanoparticles, and dissolved Al3+ also contributes to the toxic effects.